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Organization and expression of mouse Hox3 cluster genes
J Goto1, T Miyabayashi, Y Wakamatsu
1Department of Biochemistry, School of Medicine, University of Tokyo, Japan.
Molecular & General Genetics : MGG
|May 1, 1993
Summary
Researchers mapped mouse Hox3 homeobox genes, identifying a new sequence (Hox3.5). Gene expression patterns during embryogenesis suggest positional collinearity within the Hox3 cluster, similar to other Hox clusters.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- Hox genes are crucial for establishing the anterior-posterior axis during embryonic development.
- Understanding the organization and regulation of Hox gene clusters provides insights into developmental processes.
Purpose of the Study:
- To physically map and characterize mouse Hox3 homeobox sequences.
- To determine the genomic location of novel Hox sequences within the Hox3 cluster.
- To analyze the expression patterns of specific Hox3 genes during embryogenesis.
Main Methods:
- Analysis of overlapping genomic clones to determine physical linkage of Hox sequences.
- Mapping of Hox1.7 and Hox1.8 within the Hox1 cluster.
- Studying gene expression patterns during mouse embryogenesis.
Main Results:
- Six mouse Hox3 homeobox sequences were physically mapped, including the novel Hox3.5.
- The locations of Hox1.7 and Hox1.8 within the Hox1 cluster were defined.
- Expression patterns of Hox3.6 and Hox3.5 during embryogenesis showed collinearity with their positions in the Hox3 cluster.
Conclusions:
- The Hox3 cluster exhibits a similar spatial and temporal expression pattern to other Hox clusters.
- Physical mapping and expression analysis contribute to understanding Hox gene regulation in development.