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Updated: Sep 6, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
[Cytokines and immune response]
L Pelletier1, B Bellon, P Druet
1INSERM U28, hôpital Broussais, Paris.
Abstract:
This review paper briefly describes the principal cytokines involved in immune response, and in particular their role in some of its key-steps: antigen presentation; activation, proliferation and differentiation of CD4+ T cell subsets; antibody response by B cells; manifestations of hypersensitivity. The role played by cytokines in cytotoxic T cell (CTL) differentiation and in T cell-mediated suppression phenomena is also discussed. It seems that the auxiliary CD4+ memory cells secrete either IL-2 and interferon gamma (Th1) or IL-4 and IL-5 (Th2). It has recently been suggested that cytotoxic/suppressor CD8+ cells can also be divided into cells that secrete interferon gamma and no IL-4 (CTL) and cells that secrete IL-4 and IL-5 (suppressor T cells). The reciprocal regulatory effects of these cell populations through these interleukins is also discussed.
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