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CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay
J K Nicholson1, B M Jones, M Hubbard
1Immunology Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Cytometry
|January 1, 1993
Abstract:
We evaluated a flow cytometric method for determining the proportion of CD4-positive T lymphocytes in whole blood using a single three-color tube containing fluorochrome-labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six-tube, two-color assay gated on light scatter parameters. Excellent correlation was found between the three-color analysis using a CD45/side scatter gate and the standard two-color analysis with a light scatter gate. This single-tube three-color test is a promising assay for monitoring CD4+ T-lymphocytes.