Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superpositioning of 3-dimensional neuroanatomic data sets

E M Santori1, A W Toga

  • 1Department of Neurology, UCLA School of Medicine 90024.

Journal of Neuroscience Methods
|November 1, 1993
PubMed
Summary

Accurate 3D neuroimaging requires precise alignment of rat brain volumes. This study compared four methods, finding that using skull base landmarks and image density correlation improved alignment accuracy for group analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: How Will Aducanumab Approval Impact AD Research?

The journal of prevention of Alzheimer's disease·2021
Same author

Signal Hyperintensity on Unenhanced T1-Weighted Brain and Cervical Spinal Cord MR Images after Multiple Doses of Linear Gadolinium-Based Contrast Agent.

AJNR. American journal of neuroradiology·2019
Same author

Connectome imaging for mapping human brain pathways.

Molecular psychiatry·2017
Same author

Toward Precision and Reproducibility of Diffusion Tensor Imaging: A Multicenter Diffusion Phantom and Traveling Volunteer Study.

AJNR. American journal of neuroradiology·2016
Same author

Hot Topics in Research: Preventive Neuroradiology in Brain Aging and Cognitive Decline.

AJNR. American journal of neuroradiology·2015
Same author

FASTKD2 is associated with memory and hippocampal structure in older adults.

Molecular psychiatry·2014

Area of Science:

  • Neuroimaging
  • Comparative Anatomy
  • Image Registration

Background:

  • Superpositioning 3D digital volumes is crucial for group-level neuroimaging analysis.
  • Accurate alignment of individual brain scans is necessary for robust group evaluation.

Purpose of the Study:

  • To evaluate four distinct methods for aligning cryoplaned rat brain volumes.
  • To determine the most effective technique for achieving accurate 3D digital volume superpositioning.

Main Methods:

  • Cryoplaning of 6 rat heads and digital blockface imaging to create 3D volumes.
  • Testing landmark-based (lambda-bregma, skull sutures) and image density cross-correlation methods for alignment.
  • Assessing alignment accuracy using midsagittal, coronal, and horizontal cutplanes and landmark variability.

Related Experiment Videos

Main Results:

  • Landmark-based methods showed varying degrees of misalignment.
  • Ventral fiducials improved correlation statistics and reduced positional variability.
  • Image density cross-correlation yielded results comparable to a 4-point landmark approach.

Conclusions:

  • Skull base landmarks and image density cross-correlation offer effective strategies for 3D rat brain volume alignment.
  • Improved alignment is critical for advancing group-level neuroimaging studies.