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Fibroblast growth factors mobilize peritoneal macrophage intracellular calcium

K V Hackshaw1, Y Shi

  • 1Dept Internal Medicine, Ohio State University, Columbus, 43210.

Life Sciences
|January 1, 1994
PubMed

Insights

Fibroblast growth factors (FGF) increase intracellular calcium in macrophages, a key step in inflammation. Blocking calcium channels and inflammatory pathways significantly reduces this FGF-induced calcium response.

Area of Science:

  • Immunology
  • Cellular Biology
  • Molecular Medicine

Background:

  • Macrophages are crucial in inflammatory disease progression.
  • Their inflammatory mediator release is triggered by extracellular signals.
  • Fibroblast growth factors (FGF) are investigated for their role in inflammation.

Purpose of the Study:

  • To investigate the role of acidic and basic FGF in intracellular calcium mobilization in murine peritoneal macrophages.
  • To understand the signaling pathways involved in FGF-induced calcium responses.

Main Methods:

  • Murine peritoneal macrophages were cultured with acidic and basic FGF.
  • Intracellular calcium levels were measured using fluorometric methods.
  • Specific channel blockers and enzyme inhibitors (5-lipoxygenase, cyclooxygenase) were used to elucidate the signaling pathways.

Main Results:

  • Both acidic and basic FGF induced a rapid and significant rise in cytosolic calcium in macrophages.
  • The maximal effective dose for both FGFs was 10 ng/ml.
  • The FGF-induced calcium rise was abolished by calcium channel blockers and significantly reduced by 5-lipoxygenase and cyclooxygenase inhibitors.

Conclusions:

  • FGFs trigger intracellular calcium mobilization in macrophages, suggesting a role in inflammatory processes.
  • The calcium response is mediated through both voltage-dependent and independent calcium channels.
  • Inhibition of 5-lipoxygenase and cyclooxygenase pathways significantly impacts the FGF-induced calcium response, highlighting their involvement in FGF signaling during inflammation.

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