Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic actin structures stabilized by profilin

T Finkel1, J A Theriot, K R Dise

  • 1Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Proceedings of the National Academy of Sciences of the United States of America
|February 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantifying the roles of space and stochasticity in computer simulations for cell biology and cellular biochemistry.

Molecular biology of the cell·2020
Same author

Utility of blood pressure genetic risk score in admixed Hispanic samples.

Journal of human hypertension·2016
Same author

Cysteine mutagenesis in the voltage-dependent sodium channel structural insights and implications.

Trends in cardiovascular medicine·2011
Same author

NOS1AP variant associated with incidence of type 2 diabetes in calcium channel blocker users in the Atherosclerosis Risk in Communities (ARIC) study.

Diabetologia·2009
Same author

Polymorphisms of the tumor suppressor gene LSAMP are associated with left main coronary artery disease.

Annals of human genetics·2008
Same author

Comparison of quantitative methods for cell-shape analysis.

Journal of microscopy·2007
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Profilin, an actin monomer binding protein, regulates actin stability and distribution in cells. Overexpressing profilin increases filamentous actin concentration and alters actin network structure.

Area of Science:

  • Cell Biology
  • Biochemistry

Background:

  • Profilin is a ubiquitous protein that binds actin monomers.
  • The precise role of profilin in regulating actin dynamics in vivo is not fully understood.

Purpose of the Study:

  • To investigate the in vivo function of human profilin.
  • To assess the impact of profilin on actin dynamics and cellular architecture.

Main Methods:

  • Production and analysis of clonal cell lines with overexpressed human profilin.
  • Measurement of filamentous actin concentration and actin filament half-life.
  • Assessment of actin filament distribution using microscopy.

Main Results:

  • Increased profilin levels correlated with higher filamentous actin concentration.

Related Experiment Videos

  • Actin filament half-life was directly proportional to steady-state profilin concentration.
  • Profilin overexpression altered actin distribution, increasing submembranous actin networks and reducing parallel actin bundles.

    • Conclusions:

    • Profilin plays a critical role in regulating actin stability in vivo.
    • Profilin influences the distribution of dynamic actin structures within the cell.