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Related Experiment Videos

Vitreoretinal toxicity of basic fibroblast growth factor

H Borhani1, G A Peyman, M H Rahimy

  • 1LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112-2234.

International Ophthalmology
|August 1, 1993
PubMed
Summary

Basic fibroblast growth factor (b-FGF) shows therapeutic promise in ophthalmology but can cause sight-threatening vitreoretinal issues at higher doses. Doses up to 2 micrograms are safe, while 4 micrograms or more induce proliferative changes.

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Toxicology

Background:

  • Basic fibroblast growth factor (b-FGF) is a potent growth factor with significant therapeutic potential in ocular diseases.
  • However, b-FGF is also implicated in the development of proliferative vitreoretinal disorders.
  • Understanding its safety profile is crucial for its clinical application.

Purpose of the Study:

  • To evaluate the vitreoretinal toxicity of basic fibroblast growth factor (b-FGF) in a rabbit eye model.
  • To determine the dose-dependent effects of intravitreal b-FGF on retinal and vitreous tissues.
  • To inform the safe therapeutic use of b-FGF in ophthalmology.

Main Methods:

  • Intravitreal injections of varying doses of b-FGF were administered to rabbit eyes.

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  • Vitreoretinal toxicity was assessed through clinical observation, electroretinography (ERG), and histopathological examination.
  • Doses ranged from sub-toxic levels to those expected to induce adverse effects.

    • Main Results:

    • Intravitreal b-FGF up to 2 micrograms per eye demonstrated no significant toxicity.
    • Doses of 4 micrograms or higher induced severe, sight-threatening vitreoretinal proliferative changes.
    • Despite pathological changes like retinal folds and detachment, ERG amplitudes remained normal or increased at 8 micrograms.

    Conclusions:

    • Basic fibroblast growth factor (b-FGF) exhibits a dose-dependent toxicity profile in the rabbit vitreous.
    • Therapeutic applications of b-FGF in ophthalmology require careful dose selection to avoid adverse proliferative effects.
    • Further research is needed to balance the benefits and risks of b-FGF in retinal disease treatment.