Surface morphology following photorefractive keratectomy

To examine the effects of surface dehydration on corneal surface morphology before and after photorefractive keratectomy, corneas of fresh porcine eyes were deepithelialized and then immediately fixed or treated according to one of six protocols (four eyes per protocol): dehydrated under the illumination of an operating microscope; dehydrated and then rehydrated with topically applied balanced salt solution; photorefractive keratectomy; photorefractive keratectomy followed by surface dehydration; or photorefractive keratectomy followed by dehydration and subsequent rehydration. Surface smoothness (S) was measured with an image analysis system. The surfaces of the unablated corneas were smooth (S = 0.992 +/- 0.002), and dehydration did not significantly (P = .7) influence the morphology. After ablation, dehydration resulted in apparent fragmentation of the superficial lamellae, with consequent roughening of the surface (P = .0001). Rehydration was only partially successful in reducing surface irregularity. We conclude that corneal dehydration during and following photorefractive keratectomy roughens the corneal surface and should be carefully avoided.