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Molecular cloning of feline interleukin-6 cDNA
T Ohashi1, Y Matsumoto, T Watari
1Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
The Journal of Veterinary Medical Science
|December 1, 1993
Abstract:
We cloned and sequenced feline interleukin-6 (IL-6) cDNA using the polymerase chain reaction (PCR) technique to obtain information on regulation of the IL-6 gene. The feline IL-6 cDNA cloned in this study was 752 bp long and included all the amino acid coding region. At the amino acid level, the overall identities of the feline IL-6 to the human and murine counterparts were 58% and 39%, respectively. After transfection with the expression vector containing the cDNA, CRFK cells produced biologically active proteins that showed hybridoma growth promoting activity.