Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alternatively spliced transcripts of the human complement C2 gene

J Cheng1, J E Volanakis

  • 1Department of Medicine, University of Alabama at Birmingham 35294.

Journal of Immunology (Baltimore, Md. : 1950)
|February 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the CLSI cefoxitin 30-microg disk-diffusion method for detecting methicillin resistance in staphylococci.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2006
Same author

Analysis of candidate genes on chromosome 2 in oral cleft case-parent trios from three populations.

Human genetics·2006
Same author

Toroidal symmetry of the geodesic acoustic mode zonal flow in a tokamak plasma.

Physical review letters·2006
Same author

Comparison of transendocardial and retrograde coronary venous intramyocardial catheter delivery systems in healthy and infarcted pigs.

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions·2006
Same author

Capsaicin for allergic rhinitis in adults.

The Cochrane database of systematic reviews·2006
Same author

Association between metabolic gene polymorphisms and susceptibility to peripheral nerve damage in workers exposed to n-hexane: a preliminary study.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals·2006
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

TGF-β controls developmental fate and functional identity of thymic γδ T cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Distinguishing Th17 cells as a unique subset.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Researchers discovered novel C2 gene splice variants in human cells. These alternative splicing events in C2 mRNA can lead to truncated C2 proteins, impacting cellular function.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • The C2 gene plays a role in cellular processes, but its transcript variants are not fully understood.
  • Alternative splicing is a key mechanism for generating protein diversity from a limited number of genes.

Purpose of the Study:

  • To identify and characterize alternatively spliced C2 gene transcripts.
  • To investigate the impact of alternative splicing on C2 protein coding potential.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify C2 mRNA from HepG2 cells.
  • Cloning and nucleotide sequencing were performed to analyze C2 mRNA variants.
  • RNase protection assays confirmed the presence of splice variants.
  • RT-PCR was used to detect variants in various human cell lines.

Related Experiment Videos

Main Results:

  • Four novel C2 mRNA size variants were identified, resulting from differential splicing.
  • Specific exon deletions (exons 2 and 3, exon 3, exon 17, exons 6, 7, and part of exon 18) were observed.
  • Two variants maintained the C2 open reading frame, while two others introduced frameshifts and premature termination codons.
  • All identified variants were detected in HepG2 cells, human liver, U937, and U105-MG cells.
  • An additional variant lacking the exon 6 region was found in some cell types.

Conclusions:

  • Differential splicing of C2 gene transcripts generates multiple mRNA variants.
  • Some C2 splice variants result in truncated proteins due to frameshifts and premature stop codons.
  • These alternatively spliced C2 mRNAs are expressed across various human cell types, suggesting functional significance.