[Studies in the field of hydrolytic enzymes]

A study in the properties of hydrolytic enzymes and the processes of hydrolysis relized by them is a main trend in the department activity. Special attention is paid to the studies in hydrolases of microbial origin. Methods are developed for obtaining the proteolytic and cellulolytic complexes synthetized by the Aspergilla and Actinomyces. New, nonstudied (or little studied) individual enzymes are isolated from the proteolytic complexes proteinases form Asp. flavus, Asp. oryzae, Str. griseus; carboxypeptidase from Str. griseus. LGG-aminopeptidase from Asp. flavus. The presence of dipeptidases of different types is shown. The methods for cystallization of pepsin, alpha-chemotrypsin, trypsin, protease from Str. griseus, alpha-amylase from Asp. cryzae are developed and improved. The properties of the isolated enzymes are studied--their substrate specifity, elements, of chemical structure, effect of activators and inhibitors, metal ions, etc. Special attention is paid to studies in stability and conditions of enzymic proteins stabilization. On the basis of studies in the field of preparative enzymology as well as in stabilization and denaturation of the enzymes, seven preparations (or new methods of their production) are worked out for application to medicine. The studies in the process of gelatin hydrolysis with protease of Str. griseus made it possible to develop a new technique for silve regeneration by means of the preparation "proteinase-1". The enzymic-antibiotic preparation "protezym" proved to be effective when feeding sucking pigs and broilers.