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Related Experiment Videos

Tail length determination in bacteriophage T4

N K Abuladze1, M Gingery, J Tsai

  • 1Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.

Virology
|March 1, 1994
PubMed
Summary

Bacteriophage T4 gene 29 protein acts as a molecular ruler, directly controlling tail length. Altering the gene 29 protein

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Area of Science:

  • Molecular biology
  • Virology
  • Genetics

Background:

  • Bacteriophage T4 tail length determination is not fully understood.
  • Previous studies suggested gene 29 protein's involvement as a template.
  • Gene H protein in bacteriophage lambda serves as a similar proposed model.

Purpose of the Study:

  • To investigate the role of bacteriophage T4 gene 29 protein in tail length regulation.
  • To determine if gene 29 protein functions as a molecular ruler or template.

Main Methods:

  • Constructing deletions and duplications in the bacteriophage T4 gene 29.
  • Cloning modified gene 29 into the pET-11d expression vector.
  • Performing complementation studies with engineered plasmids.

Main Results:

  • Tail length is proportionally altered by changes in gene 29 protein length.
  • C-terminal deletions decrease tail length; duplications increase it.
  • N-terminal or middle deletions result in inactive protein and non-viable phage.

Conclusions:

  • Bacteriophage T4 gene 29 protein directly dictates tail length.
  • The gene 29 protein functions as a template or ruler during tail assembly.
  • Protein length directly correlates with bacteriophage tail length.

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