Related Experiment Video
Updated: Aug 19, 2026

Measuring Lactase Enzymatic Activity in the Teaching Lab
Published on: August 6, 2018
[Lipase activity in the calf serum under different types of feeding]
Abstract:
The development of lipase activity in serum (S-lipase activity) was studied in two groups of the calves of the Bohemian Red-Pied breed in the post-natal period up to the age of nine weeks from birth. From the eighth day of age, the two groups differed from each other in their lactic nutrition. Group A calves were given whole milk (obtained at primary production) and group B calves were fed the Laktosan mixture instead of milk. The different type of nutrition did not influence the character of the development of S-lipase activity with increasing age. A difference was found between the two groups as to the level of the activity of the enzyme under study. In the whole-milk group the average S-lipase activity values were higher than in the group fed Laktosan in a larger part of the test period. Some differences were statistically significant. It is assumed that lipase activity in blood is a reflection of the external secretion of pancreas changing according to the structure of food taken in. The activity of S-lipase was determined by the method according to Cherry and Crandall (Seligson, 1964); the average minimum value was 0.025 +/- 0.0017 u. in group A and 0.017 +/- 0.0081 u. in group B, and the average maximum value was 0.370 +/- 0.100 u. and 0.286 +/- 0.0766 u., respectively.

