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Alcoholysis of epsilon-decalactone with polyethylene glycol-modified lipase in 1,1,1-trichloroethane
M Furukawa1, Y Kodera, T Uemura
1Department of Materials Science and Technology, Toin University of Yokohama, Japan.
Biochemical and Biophysical Research Communications
|February 28, 1994
Abstract:
Lipase from Pseudomonas cepacia was modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine, activated PEG2, to form PEG-lipase. The PEG-lipase is soluble and active in organic solvents. It catalyzes alcoholysis of racemic epsilon-decalactone with ethanol in 1,1,1-trichloroethane to form (R)-hydroxydecanoic acid ethyl ester. No alcoholysis of (S)-decalactone takes place. These results were discussed in relation to carbon number of n-alcohol, optimum temperature and comparison with modified and non-modified lipases.