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An electrophoretic procedure to detect three key enzymes of the ascorbate system
M De Tullio1, C Paciolla, L De Gara
1Istituto Botanico, Università di Bari.
Abstract:
A method for detection of ascorbic acid oxidase, ascorbic acid peroxidase and dehydroascorbic acid reductase is reported. This method allows the qualitative determination of the presence of these enzymes, also in conditions where the commonly used spectrophotometric assays are unreliable.