Related Experiment Videos
Rapid assay of iopanoic acid in dog plasma using a Hisep column
A M Andeejani1, H Hughes, D M Feuchuk
1Radiology Department, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Biomedical Chromatography : BMC
|January 1, 1994
Abstract:
A rapid method for the determination of iopanoic acid (IOP) in dog plasma utilizing a Hisep column was developed. A mobile phase of 12% methanol, 88% 0.05 M phosphate buffer pH 3.4 yielded a k' of 8.5 with no interference from proteins present in plasma. Recoveries of IOP from spiked plasma ranged from 97% to 103% at 270 mumol/L and 1.75 mmol/L respectively. Replication was +/- 2.8% at 1.75 mmol/L and +/- 6% at 21 mumol/L. A method utilizing 2,4,6-triiodobenzoic acid as internal standard was also developed for comparison.