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Electrophoretically mediated microanalysis of ethanol

B J Harmon1, D H Patterson, F E Regnier

  • 1Purdue University, Department of Chemistry, West Lafayette, IN 47907-1393.

Journal of Chromatography. A
|December 31, 1993
PubMed
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Electrophoretically mediated microanalysis (EMMA) offers a miniaturized capillary electrophoresis method for ethanol determination. This technique provides accurate results comparable to spectrophotometric methods, with advantages in speed and sample volume.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Accurate ethanol determination is crucial in various scientific and industrial applications.
  • Traditional methods may lack efficiency or require larger sample volumes.
  • Electrophoretic techniques offer potential for miniaturized and rapid analyses.

Purpose of the Study:

  • To develop and validate a capillary electrophoresis method for ethanol quantification using electrophoretically mediated microanalysis (EMMA).
  • To compare the EMMA method's performance against a standard spectrophotometric procedure for ethanol determination.

Main Methods:

  • Ethanol was quantified using capillary electrophoresis with electrophoretically mediated microanalysis (EMMA).
  • The method employed alcohol dehydrogenase for ethanol oxidation and monitored NAD+ reduction to NADH at 340 nm.

Related Experiment Videos

  • Quantification involved an internal standard and normalization for peak migration time.

    • Main Results:

    • The EMMA method achieved a relative standard deviation (R.S.D.) of 2.7% for ethanol determination.
    • The linear range was determined by the reaction time prior to detection.
    • No significant difference was observed when comparing EMMA results to the Sigma spectrophotometric procedure.

    Conclusions:

    • EMMA provides a reliable and accurate method for ethanol determination via capillary electrophoresis.
    • The technique offers advantages of electrophoretic mixing and miniaturization.
    • EMMA is a viable alternative to conventional spectrophotometric methods for ethanol analysis.