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Determination of blood serum protein reactivity in children with behavior disturbances
Insights
Children with behavior disturbances exhibit altered serum proteins, including reduced serotonin binding and increased cationic activity, suggesting electrolyte imbalances affecting autonomic functions.
Area of Science:
- Biochemistry
- Pediatrics
- Neuroscience
Background:
- Serum protein analysis offers insights into physiological states.
- Behavioral disturbances in children can be linked to underlying biochemical changes.
Purpose of the Study:
- To investigate differences in serum cationic state and protein characteristics between children with and without behavior disturbances.
- To explore potential links between these biochemical markers and autonomic function integration.
Main Methods:
- Utilized an original method for determining serum cationic state using heparin exchange chromatography.
- Assessed protein reactivity, electrophoretic mobility of serum proteins at pH 7.4, total sodium, and total serum proteins.
Main Results:
- Children with behavior disturbances showed a reduced number of serotonin-binding sites on serum proteins.
- A higher binding affinity between serotonin and proteins was observed in this group.
- Serum proteins in children with behavioral issues exhibited lower resistance to denaturation, leading to increased cationic activity and electrophoretic mobility.
Conclusions:
- Serum protein alterations in children with behavior disturbances suggest underlying perturbations in electrolyte exchange, particularly sodium.
- These findings may indicate dysregulation in the integration centers of autonomic functions.
Abstract:
The researches were carried out on two groups of children: normals and with behavior disturbances. The serum cationic state was determined by an original method, using heparin as cautions exchanger in physiological pH, and the protein reactivity; the electrophoretic mobility of serum proteins at pH 7.4; total soduim and serum proteins. The obtained results show that serum proteins in children with behavior disturbances differ from those of normals, having: 1) smaller number of serotonin-fixing sites, 2) higher strength interaction between the serotonin and proteins, 3) lower resistance to the denaturant agents which has as a consequence the increase of cationic activity in the serum, and an increased electrophoretic mobility. The data are discussed and interpreted as perturbations in the electrolytes exchange (Na+, especially) in the integration centres of the autonomic functions.