Analysis of derivatized peptides by capillary electrophoresis

Thirteen synthetic prothrombin leader peptides differing only in C terminus were analyzed by capillary electrophoresis. These peptides were derivatized using the novel, fluorescent derivatizing agent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Derivatized peptides were detected using fluorescence and ultraviolet absorption signals, and underivatized peptides were detected using ultraviolet absorbance at 185 nm. Data are presented which compare the analysis of the derivatized and underivatized peptides.