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[Plasmid profiles of 120 strains of Pseudomonas aeruginosa]

T Wu1

  • 1Nanjing Railway Medical College.

Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|October 1, 1993
PubMed

Insights

Plasmid profiling of Pseudomonas Aeruginosa (PA) clinical isolates revealed diverse plasmid types. Comparing plasmid profiles with serotypes aids in the epidemiological fingerprinting of PA strains.

Area of Science:

  • Microbiology
  • Epidemiology
  • Molecular Biology

Background:

  • Pseudomonas Aeruginosa (PA) is a significant opportunistic pathogen.
  • Understanding the genetic diversity of PA clinical isolates is crucial for infection control.
  • Plasmid carriage can influence bacterial virulence and antibiotic resistance.

Purpose of the Study:

  • To characterize the plasmid profiles of clinical isolates of Pseudomonas Aeruginosa from Nanjing City.
  • To compare plasmid profiling with serotyping as an epidemiological typing method for PA.

Main Methods:

  • Plasmid DNA was extracted from 120 clinical PA isolates using the Kado and Liu method.
  • Plasmid profiles were analyzed, and sizes were determined.
  • Serotyping was performed, and results were compared with plasmid profiles.

Main Results:

  • Only 24.2% of the PA strains harbored plasmids.
  • Thirteen distinct plasmid profiles were identified, with sizes ranging from 1.91 to 45.14 MDa.
  • Serotyping achieved 95.00% typability.

Conclusions:

  • Plasmid profiling provides valuable genetic information for PA isolates.
  • Combining plasmid profiling and serotyping may enhance the epidemiological fingerprinting of clinical PA.
  • Further investigation into the role of plasmids in PA pathogenicity is warranted.

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