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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
Published on: June 10, 2015
Expression and stability of recombinant RQ-mRNAs in cell-free translation systems
V I Ugarov1, Morozov IYu, G Y Jung
1Institute of Protein Research, Russian Academy of Sciences, Puschino, Moscow Region.
Abstract:
Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.
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