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Genetic analysis of 63 mutations affecting maize kernel development isolated from Mutator stocks
M J Scanlon1, P S Stinard, M G James
1Department of Biochemistry, Iowa State University, Ames 50011.
Genetics
|January 1, 1994
Summary
Researchers identified 63 maize kernel development mutations using Robertson's Mutator stocks, defining 14 new gene loci. These defective kernel (dek) mutations offer opportunities for molecular cloning via transposon tagging.
Area of Science:
- Plant genetics
- Molecular biology
- Maize genetics
Background:
- Robertson's Mutator (Mu) stocks are active transposon systems used in maize genetics.
- Mutations affecting kernel development are crucial for understanding maize growth and yield.
Purpose of the Study:
- To identify and characterize novel mutations affecting maize kernel development.
- To define new maize gene loci and map their locations.
- To assess the potential for molecular cloning of these genes using transposon tagging.
Main Methods:
- Isolation of mutations from active Robertson's Mutator (Mu) stocks.
- Genetic mapping of defective kernel (dek) mutations to chromosome arms.
- Allelism testing to identify relationships between mutations.
- Viability testing of homozygous mutants to assess pleiotropic effects.
Main Results:
- Sixty-three mutations affecting maize kernel development were isolated.
- Fourteen previously undescribed maize gene loci were defined.
- Fifty-three dek mutations were mapped to chromosome arms, with 21 precisely mapped.
- Twenty instances of allelism were identified, with phenotypic variability noted in three series.
- Numerous dek mutations showed pleiotropic effects on plant development.
Conclusions:
- The study identified a significant number of novel maize gene loci affecting kernel development.
- The use of Mu transposon tagging facilitates molecular cloning of these affected genes.
- These findings provide a foundation for further research into maize kernel development and gene function.