Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Artifacts in computational optical-sectioning microscopy

J G McNally1, C Preza, J A Conchello

  • 1Biomedical Computer Laboratory, Washington University, St. Louis, Missouri 63110.

Journal of the Optical Society of America. A, Optics, Image Science, and Vision
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inherited palmoplantar keratodermas: the heart of the matter.

Clinical and experimental dermatology·2017
Same author

Male genital lichen sclerosus in recipients of bone marrow transplants.

Clinical and experimental dermatology·2016
Same author

A study of liver function following massive arsenotherapy for syphilis.

American journal of syphilis, gonorrhea, and venereal diseases·2010
Same author

Recent trends in the therapy of liver disease.

The Medical annals of the District of Columbia·2010
Same author

Black fly incubator-aerator cabinet.

Science (New York, N.Y.)·2010
Same author

New pseudophyllidean cestodes from the Great Lakes region; Diphyllobothrium oblongatum n. sp. from gulls.

The Journal of parasitology·2010
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
See all related articles

This study validates an optical model for optical-sectioning microscopy, finding it accurate for reconstructing 3D microscopy images. Researchers identified a novel artifactual elongation and suggest modifications for improved image reconstruction.

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Image Processing

Background:

  • Computational optical-sectioning microscopy relies on accurate optical models for image reconstruction.
  • Understanding the point-spread function (PSF) is crucial for correcting image distortions in 3D microscopy.

Purpose of the Study:

  • To evaluate the accuracy of the most complete optical model for computational optical-sectioning microscopy.
  • To investigate image reconstruction artifacts and their causes in nonconfocal 3D microscopy.
  • To propose solutions for improving image quality and overcoming inherent limitations.

Main Methods:

  • Experimental validation of theoretical point-spread functions (PSFs) under diverse imaging conditions.
  • Application of linear restoration methods to reconstruct images of a 10-micron fluorescent bead phantom.

Related Experiment Videos

  • Analysis of artifactual axial elongation and its relationship to the null space in 3D microscopy.

    • Main Results:

    • Excellent agreement was observed between experimental and theoretical PSFs.
    • Linear restoration closely reproduced theoretically optimal reconstructions, but revealed an artifactual axial elongation.
    • Image quality degraded with depth, necessitating depth-specific PSF determination for accurate reconstruction.

    Conclusions:

    • The optical model for optical sectioning demonstrates high accuracy.
    • Reconstruction methods must adapt to PSF shift variance if optical adjustments are insufficient.
    • Addressing artifacts from the missing cone of frequencies requires advanced microscopical or nonlinear algorithmic techniques.