Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrin expression by human articular chondrocytes

V L Woods1, P J Schreck, D S Gesink

  • 1University of California, San Diego Medical Center 92103-8417.

Arthritis and Rheumatism
|April 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preinterventional Third-Molar Assessment Using Robust Machine Learning.

Journal of dental research·2023
Same author

Defective chaperone-mediated autophagy is a hallmark of joint disease in patients with knee osteoarthritis.

Osteoarthritis and cartilage·2023
Same author

Suppression of circadian clock protein cryptochrome 2 promotes osteoarthritis.

Osteoarthritis and cartilage·2020
Same author

Molecular subtype, biological sex and age shape melanoma tumour evolution.

The British journal of dermatology·2020
Same author

First Report of Cucumber Mosaic Virus in Eryngium amethystinum, Canna spp., and Aquilegia hybrids in Ohio.

Plant disease·2019
Same author

The role of the T3 molecular complex in antigen recognition and subsequent activation events.

Immunology today·2014
Same journal

Proceedings of the Annual Meeting of the American Rheumatism Association.

Arthritis and rheumatism·2020
Same journal

The regional distribution of rheumatologists: what can we do, what should we do?

Arthritis and rheumatism·2013
Same journal

Regional distribution of adult rheumatologists.

Arthritis and rheumatism·2013
Same journal

Clinically significant renal involvement in primary Sjögren's syndrome: clinical presentation and outcome.

Arthritis and rheumatism·2013
Same journal

Headache in systemic lupus erythematosus: results from a prospective, international inception cohort study.

Arthritis and rheumatism·2013
Same journal

Association of low baseline levels of erythrocyte folate with treatment nonresponse at three months in rheumatoid arthritis patients receiving methotrexate.

Arthritis and rheumatism·2013
See all related articles

Normal human articular chondrocytes express key integrin heterodimers, including alpha 1 beta 1, alpha 5 beta 1, and alpha v beta 5. These findings are crucial for understanding cartilage health and joint disease.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Immunology

Background:

  • Integrins are crucial cell surface receptors involved in cell adhesion and signaling.
  • Articular chondrocytes play a vital role in maintaining cartilage homeostasis.
  • Dysregulation of chondrocyte function is implicated in joint diseases.

Purpose of the Study:

  • To comprehensively analyze the integrin forms expressed by normal human articular chondrocytes.
  • To identify specific integrin heterodimers present on chondrocytes.
  • To investigate the distribution of integrins within cartilage layers.

Main Methods:

  • Utilized a panel of integrin isoform-specific monoclonal antibodies (MAb).
  • Employed in situ immunohistochemistry, indirect immunofluorescence, and flow cytometry.

Related Experiment Videos

  • Performed immunoprecipitation followed by SDS-PAGE analysis.

    • Main Results:

    • Identified alpha 5, alpha v, and beta 1 integrin subunits and alpha v beta 3 and alpha v beta 5 heterodimers.
    • Detected alpha 1 beta 1, alpha 5 beta 1, and alpha v beta 5 heterodimers prominently.
    • Observed increased expression of alpha v-containing integrins on superficial chondrocytes.
    • Confirmed presence of alpha 3 beta 1 integrin.

    Conclusions:

    • Normal human articular chondrocytes express substantial amounts of alpha 1 beta 1, alpha 5 beta 1, and alpha v beta 5 integrins.
    • Lesser quantities of alpha 3 beta 1 and alpha v beta 3 integrins were also detected.
    • Integrin expression varies with chondrocyte depth, with superficial cells showing more alpha v-containing integrins.
    • These findings provide a foundation for studying chondrocyte integrin roles in cartilage homeostasis and joint disease pathogenesis.