Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelin in human congestive heart failure

C M Wei1, A Lerman, R J Rodeheffer

  • 1Cardiorenal Research Laboratory, Mayo Clinic and Foundation, Rochester, MN 55905.

Circulation
|April 1, 1994
PubMed
Summary

In severe congestive heart failure (CHF), elevated plasma endothelin (ET) is mainly the precursor big-ET, not the active ET-1. Cardiac ET remains ET-1 and unchanged, with plasma ET correlating to CHF severity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reassessing the Carotid Artery Plaque "Rim Sign" on CTA: A New Analysis with Histopathologic Confirmation.

AJNR. American journal of neuroradiology·2022
Same author

Why Not Dipyridamole: a Review of Current Guidelines and Re-evaluation of Utility in the Modern Era.

Cardiovascular drugs and therapy·2021
Same author

Structural and electronic properties of C<sub>60</sub> fullerene network self-assembled on metal-covered semiconductor surfaces.

The Journal of chemical physics·2021
Same author

[Prevalence and predictors of intraplaque hemorrhage in stroke patients with intracranial atherosclerosis].

Zhonghua yi xue za zhi·2020
Same author

The long non-coding RNA PVT1/miR-145-5p/ITGB8 axis regulates cell proliferation, apoptosis, migration and invasion in non-small cell lung cancer cells.

Neoplasma·2020
Same author

A Metabolic Intravascular Platform to Study FDG Uptake in Vascular Injury.

Cardiovascular engineering and technology·2020

Area of Science:

  • Cardiovascular Research
  • Biochemistry
  • Molecular Biology

Background:

  • Plasma endothelin (ET) elevation in congestive heart failure (CHF) is reported, but its molecular form (ET-1 vs. big-ET) and myocardial involvement are unclear.
  • It is unknown if ET levels increase in early CHF stages (asymptomatic left ventricular dysfunction) and correlate with ventricular dysfunction severity.

Purpose of the Study:

  • To investigate plasma and cardiac tissue endothelin (ET) concentrations and molecular forms in healthy individuals and CHF patients across New York Heart Association (NYHA) classes I-IV.
  • To determine the relationship between circulating ET levels and the severity of cardiac dysfunction and functional class in CHF.

Main Methods:

  • Utilized cardiac radionuclide angiogram, myocardial biopsy, radioimmunoassay, gel permeation chromatography (GPC), and immunohistochemical staining (IHCS).

Related Experiment Videos

  • Analyzed plasma and cardiac tissue samples from healthy subjects and CHF patients (NYHA class I-IV).

    • Main Results:

    • Plasma ET increased significantly in moderate (NYHA III) and severe (NYHA IV) CHF, correlating negatively with left ventricular ejection fraction and positively with functional class.
    • In severe CHF, plasma ET comprised both mature ET-1 and its precursor big-ET, with big-ET being predominant; cardiac ET remained ET-1 and was present in both healthy and failing hearts.
    • Circulating ET elevation was specific to severe CHF and correlated with hemodynamic alterations and functional class.

    Conclusions:

    • Elevated plasma ET in severe CHF is primarily due to the precursor big-ET, not mature ET-1.
    • Cardiac endothelin (ET) is present in both healthy and failing hearts as ET-1 and its activity is unchanged in CHF.
    • Increased plasma ET is characteristic of severe CHF and correlates with disease severity and hemodynamic impairment.