[Study of clearance and distribution of 125I-labeled apolipoprotein CIII in mice]

Using 125I-labeled human apoCIII, the clearance and distribution of 125I-apoCIII were investigated. Human apoCIII was purified from plasma VLDL. 125I-apoCIII was prepared by chloramine T method, The fraction clearance rate (FCR) value of 125I-apoCIII in mice plasma was 0.19/h with a plasma half life of 3 h. The radioactivity in different organs after the venous injection of 125I-apoCIII exhibited that the liver contained radioactivity 3-10 times as much as the reactivity in other organs, expressed by both the whole organ and 100 mg of tissues. Results show that there might occur some specific binding sites for apoCIII on liver cells.