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Immunoregulatory characteristics of the in vitro anti-ssDNA response
C C Anderson1, A Panoskaltsis, N R Sinclair
1Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Continuous blockade of B-cell antigen receptors (BCRs) with Fab alpha sIg prevents the anti-ssDNA response of high, but not low, density B cells. Signaling via the BCRs, by prior exposure to crosslinking F(ab')2 alpha sIg, had no effect on the spontaneous anti-DNA response, but prevented a lipopolysaccharide-induced anti-DNA response. Pretreatment with intact alpha sIg, which provides exogenously derived Fc signals, reduced the response. An Fc-signal-blocking agent, F(ab')2 anti-IgG-Fc antibody, increased the number of anti-DNA antibody-forming cells produced in the absence of exogenous IgG anti-ssDNA antibody. Thus, activation is dependent on the availability of the BCRs, prior BCR crosslinking does not interfere with activation, and endogenous IgG anti-ssDNA antibody limits the activation of anti-ssDNA-specific B cells most of which are T-cell independent. These results indicate that the anti-ssDNA response is driven through the BCR.
Continuous blockade of B-cell antigen receptors (BCRs) with Fab alpha sIg prevents the anti-ssDNA response of high, but not low, density B cells. Signaling via the BCRs, by prior exposure to crosslinking F(ab')2 alpha sIg, had no effect on the spontaneous anti-DNA response, but prevented a lipopolysaccharide-induced anti-DNA response. Pretreatment with intact alpha sIg, which provides exogenously derived Fc signals, reduced the response. An Fc-signal-blocking agent, F(ab')2 anti-IgG-Fc antibody, increased the number of anti-DNA antibody-forming cells produced in the absence of exogenous IgG anti-ssDNA antibody. Thus, activation is dependent on the availability of the BCRs, prior BCR crosslinking does not interfere with activation, and endogenous IgG anti-ssDNA antibody limits the activation of anti-ssDNA-specific B cells most of which are T-cell independent. These results indicate that the anti-ssDNA response is driven through the BCR.