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Updated: Aug 13, 2026

High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
[Application of the time-resolved immunofluorometric assay in the detection of viruses]
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing.
Abstract:
Two systems of time-resolved immunofluorometric assay (TR-IFMA), i.e., LKB system detection of viruses. The viruses detected were mainly adenovirus, influenza virus, parainfluenza virus, respiratory syncytial virus rotavirus, hepatitis B virus, human immunodeficiency virus, rubella virus, etc. Compared with methods of ELISA and RIA, TR-IFMA has the merits of high sensitivity, specificity and consistency with clinical diagnosis. The low detection limit of virus antigen by TR-IFMA is between 10-100 pg. The application of dual-label TR-IFMA also showed good effect. TR-IFMA can also be used in the detection of viral nucleic acids. The target region detected may reach the level of pg.
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