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Antigenic differences within Actinobacillus pleuropneumoniae serotype 1

R A Jolie1, M H Mulks, B J Thacker

  • 1Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824.

Veterinary Microbiology
|February 1, 1994
PubMed
Summary
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Two Actinobacillus pleuropneumoniae serotype 1 subtypes, 1A and 1B, were identified using coagglutination. Antigenic differences, particularly in LPS O-antigens, distinguish these subtypes.

Area of Science:

  • Veterinary Microbiology
  • Bacteriology
  • Immunology

Background:

  • Actinobacillus pleuropneumoniae (APP) causes significant respiratory disease in swine.
  • APP serotype 1 is a common cause of pleuropneumonia.
  • Subtyping of APP serotype 1 is crucial for accurate diagnosis and control.

Purpose of the Study:

  • To differentiate antigenic subtypes within Actinobacillus pleuropneumoniae serotype 1.
  • To investigate the antigenic basis for subtype differentiation.
  • To compare immune responses to different APP serotype 1 subtypes.

Main Methods:

  • Coagglutination (Co-A) testing of field isolates.
  • Preparation and use of absorbed hyperimmune rabbit sera.
  • Outer membrane (OM) and lipopolysaccharide (LPS) analysis via SDS-PAGE and immunoblots.

Related Experiment Videos

  • Dot-immunobinding assays.

    • Main Results:

    • Two distinct subtypes of APP serotype 1, designated 1A and 1B, were identified.
    • Antigenic differences were located in the high molecular weight region of outer membranes (40-100 kDa).
    • Lipopolysaccharide (LPS) O-antigens showed distinct patterns, with 1B LPS being different from 1A, 9, and 11.

    Conclusions:

    • APP serotype 1 can be divided into at least two antigenic subtypes (1A and 1B).
    • Antigenic differences are partly located within the LPS O-antigens.
    • APP 1B LPS shares similarities with APP 9 LPS, suggesting potential cross-reactivity or shared epitopes.