[Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer]

A relatively simple procedure was developed for estimation of the glutathione reductase activity using half-automatic analyzer. The procedure involved: preparation of hemolysates with minimal rate of erythrocyte destruction; substitution of buffers with high ionic strength for 0.05 M Tris-HCl buffer not containing EDTA. Most effective period of preincubation was found; dependence of the reaction rate on FAD was studied. The procedure developed was approved using two types of half-automatic analyzer and the model FP-901 was shown to be the most suitable for estimation of the glutathione reductase holoenzyme activity.