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Qualitative and quantitative detection of HIV-1 RNA by nucleic acid sequence-based amplification
B van Gemen1, T Kievits, P Nara
1Organon Teknika, Boxtel, The Netherlands.
AIDS (London, England)
|November 1, 1993
Abstract
Aim:
To develop a method to detect HIV-1 viral RNA by amplifying a specific nucleic acid sequence.
Method:
The nucleic acid sequence-based amplification (NASBA) method uses the simultaneous activity of avian myeloblastosis virus reverse transcriptase, T7 RNA polymerase and RNase H to amplify a specific nucleic acid target sequence.
Validation:
An in vitro cultured HIV-1 stock solution was used to validate the NASBA method and determine the variation in RNA measurement.
Conclusion:
Although NASBA is theoretically capable of specific amplification of RNA or DNA, it is most suitable for amplification of RNA, and therefore for detection of HIV-1 viral RNA.