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[Developmental changes in protein secretion in rat whole saliva]
1Department of Pedodontics, School of Dentistry, Tokyo Medical and Dental University.
Summary
Salivary protein composition changes during rat development. A specific 35 kDa protein, identified as DNase I, significantly increased with age, indicating developmental shifts in oral fluid proteins.
Area of Science:
- Oral biology
- Biochemistry
- Developmental biology
Context:
- Oral cavity conditions influence tissue development.
- Saliva plays a key role in oral development.
- Understanding salivary protein dynamics is crucial for developmental studies.
Purpose:
- To investigate proportional changes in salivary proteins during rat development.
- To correlate salivary protein composition with age-related changes.
- To identify specific proteins that change in proportion during development.
Summary:
- Saliva was collected weekly from rats aged 3-12 weeks.
- Proteins were analyzed using SDS-PAGE and silver staining.
- Total protein secretion increased with age, with inconsistent proportional changes in various proteins.
- A 35 kDa protein band, identified as DNase I, showed a prominent increase.
Impact:
- Reveals quantitative shifts in salivary protein composition during development.
- Highlights the developmental significance of specific salivary proteins like DNase I.
- Provides insights into the role of saliva in oral tissue maturation.