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trkC-like immunoreactivity in the primate descending serotoninergic system
U Arvidsson1, M Risling, J Frisén
1Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
The European Journal of Neuroscience
|February 1, 1994
Summary
Researchers investigated neurotrophin-3 receptor (trkC) distribution in monkey spinal cords. This receptor
Area of Science:
- Neuroscience
- Spinal Cord Research
- Neurotrophin Signaling
Background:
- Spinal motoneurons exhibit plasticity, influenced by various signaling substances.
- Neurotrophin-3 (NT-3) is a key neurotrophic factor implicated in neuronal development and function.
- The trkC receptor is the primary mediator of NT-3 signaling.
Purpose of the Study:
- To map the distribution of trkC-like immunoreactivity in the adult macaque monkey spinal cord.
- To investigate the relationship between trkC and serotonergic systems within the spinal cord.
Main Methods:
- Indirect immunofluorescence was employed using a specific rabbit polyclonal antibody against mouse trkC.
- The antibody targeted the carboxy-terminal domain, ensuring detection of full-length signal-transducing receptors.
- Double-labeling experiments were conducted to assess co-localization with 5-hydroxytryptamine (serotonin).
Main Results:
- trkC-immunoreactive fibers and varicosities were present throughout all spinal cord levels.
- The densest innervation was observed in the intermediolateral and Onuf's nuclei, with significant input to somatic motoneuron pools.
- Co-localization of trkC and serotonin was high in most areas, excluding the dorsal horn.
Conclusions:
- Neurotrophic signaling via trkC influences both serotonergic and non-serotonergic inputs to the adult monkey spinal cord.
- These findings suggest a role for NT-3 signaling in modulating spinal cord function and plasticity.