[Immunohistochemical detection of estrogen receptors (ER) in endometrial carcinoma]

Immunohistochemical localization of estrogen receptors (ER) using specific monoclonal antibody to human ER, H222, with ABC methods were performed on frozen sections of 22 endometrial carcinoma specimens. Specific nuclear staining of ER was observed in most epithelial, stromal and malignant cells while specific cytoplasmic staining was not observed. ER positive staining (H-Score > 75) was found in 16 of 22 cases (72.7%), and the H-Score of cancer components was significantly associated with the histological grade of endometrial carcinoma (P < 0.05). The data suggest that ER content of endometrial carcinoma reflects the degree of cancer cell differentiation and may be taken as a guideline for hormone therapy.