[Two high spontaneous lung and lymph node metastasis models derived from 615 mouse pulmonary adenocarcinoma...

The primary donor tumor of these 2 models was a spontaneous pulmonary adenocarcinoma which was originally developed in a 23-month-old male 615 mouse in our laboratory. That mouse was sacrificed for s.c. transplantation to 2 isogeneic hosts on November 12, 1988. A total of 50 generations for diffuse type and 30 generations for nodule type have since succeeded. Nodule type: The incidence of serial s.c. transplantation of this type of tumor was 100% with a latency period of 4-7d, and the lifetime of the tumor-bearing mice was about 44d. Spontaneous metastatic rate in the lungs was 100% from the 1st to the 20th generations. Diffuse type: The incidence of the s.c. transplantation tumor was 100%. The latency period was 3-6 d and the lifetime of the tumor-bearing mice was about 22 d. The spontaneous metastatic rate in the lungs and lymph nodes (after the 7th generation) was 100% from the 1st through the 35th generations. The origin of these 2 types was the same and their morphological features were similar. But several of their biological characteristics (including drug sensitivity and s.c. transplantation tumor growth speed) were different.