Related Experiment Video
Updated: Aug 23, 2026

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
Published on: June 2, 2014
Molecular mechanisms of olfactory neuronal gene regulation
1Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21205.
Abstract:
The mammalian olfactory system utilizes a biochemical cascade mediated by specialized proteins to detect odorants with high sensitivity and specificity. The recent identification of olfactory neuron-specific components for each step in the signalling cascade suggests that expression of these proteins is coordinately controlled by cis-acting regulatory sequences and trans-acting transcriptional activators. We have used molecular genetic methods to characterize sequences encoding tissue-specific DNA-binding sites in the genes for components of the odorant transduction pathway and have identified a putative transcription factor, Olf-1, that functions at these sites to regulate gene expression.
More Related Videos
10:16Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
Published on: July 13, 2015
06:32Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
Published on: June 5, 2017
Related Concept Videos
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Olfaction
The olfactory receptors are embedded in the cilia of the...
RNA Polymerase II Accessory Proteins
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Regulation of Expression at Multiple Steps