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Method-dependent variations in the stability of apolipoprotein B in a stabilized liquid reference material
J V Mei1, M K Powell, L O Henderson
1Division of Environmental Health Laboratory Sciences, Centers for Disease Control and Prevention, Atlanta, GA 30341-3724.
Clinical Chemistry
|May 1, 1994
Summary
This study assessed the storage stability of human apolipoprotein B (apo B) reference material. Results show apo B degrades over time, with stability varying significantly based on storage temperature and the immunoassay method used for quantification.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Immunology
Background:
- Accurate quantification of human apolipoprotein B (apo B) is crucial for clinical diagnostics.
- Standardized reference materials are essential for reliable immunoassay performance.
- The International Federation of Clinical Chemistry developed a stabilized liquid-frozen reference material (SLRM) for apo B.
Purpose of the Study:
- To evaluate the storage life and thermal stability of the apo B SLRM.
- To assess the impact of different storage temperatures and durations on apo B integrity.
- To compare apo B quantification using various immunological methods.
Main Methods:
- Accelerated Arrhenius-type temporal studies (short-term and long-term) were conducted.
- Storage stability was assessed at -70°C and 4°C.
- Quantification methods included RIA, radial immunodiffusion, ELISA, and nephelometry.
Main Results:
- Immunoreactive apo B showed significant degradation with increasing temperature and time.
- The SLRM remained stable for up to 1 year at -70°C.
- Storage at 4°C for 10 months resulted in <10% decline in immunoreactive apo B.
- RIA, radial immunodiffusion, and ELISA showed similar losses of immunoreactive apo B.
- Nephelometry overestimated apo B amounts, particularly in aged samples.
Conclusions:
- The apo B SLRM's measured stability is dependent on the immunoassay method employed.
- Different immunological techniques yield varying results for apo B quantification in thermally aged reference materials.
- Careful selection of quantification methods is necessary for accurate apo B assessment.