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[Perioperative alterations in polymorphonuclear leukocyte function mediated by protein kinase C]
K Yokota1, T Nishihira, R Shineha
1Second Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Nihon Geka Gakkai Zasshi
|March 1, 1994
Summary
Surgical stress alters polymorphonuclear leukocyte (PMN) function. Protein kinase C activation suppresses NADPH oxidase but enhances the myeloperoxidase system, impacting microbicidal activity differently based on stress severity.
Area of Science:
- Immunology
- Surgical Oncology
- Cellular Biology
Background:
- Polymorphonuclear leukocytes (PMNs) are crucial for innate immunity.
- Surgical stress can significantly impact immune cell function.
- Protein kinase C (PKC) plays a role in cellular signaling pathways.
Purpose of the Study:
- To investigate perioperative changes in PMN function.
- To elucidate the role of protein kinase C in mediating these alterations.
- To assess the impact of surgical stress severity on PMN responses.
Main Methods:
- Studied 26 patients undergoing gastrointestinal surgery (esophagectomy or cholecystectomy).
- Measured PMN superoxide anion (O2-) production stimulated by phorbol 12-myristate 13-acetate (PMA) as an indicator of NADPH oxidase activity.
- Assessed myeloperoxidase-H2O2-halide system activity using luminol-dependent chemiluminescence.
Main Results:
- Postoperative O2- production was suppressed, reaching a minimum on Postoperative Day 3 (POD 3).
- Luminol-dependent chemiluminescence significantly increased after surgery, peaking on POD 3.
- These functional changes in PMNs were more pronounced in patients who underwent severe surgical stress (esophagectomy).
Conclusions:
- Postoperative PKC activation differentially affects PMN microbicidal activity.
- PKC signaling appears to suppress NADPH oxidase activity while activating the myeloperoxidase system.
- The degree of surgical stress influences the extent of these PMN functional alterations.