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Interaction of poliovirus with its cell surface binding site
J A Bibb1, G Witherell, G Bernhardt
1Department of Microbiology, SUNY at Stony Brook 11794-8621.
Virology
|May 15, 1994
Summary
Poliovirus binding to HeLa cells was studied using a silicon oil partition assay. Both virulent and avirulent poliovirus strains exhibit similar binding affinities and rates to host cells.
Area of Science:
- Virology
- Molecular Biology
- Biophysics
Background:
- Poliovirus interacts with specific cellular receptors to initiate infection.
- Understanding these interactions is crucial for developing antiviral strategies and vaccines.
Purpose of the Study:
- To characterize the binding kinetics and affinity of poliovirus to cellular receptors.
- To compare the binding characteristics of virulent and avirulent poliovirus strains.
Main Methods:
- Receptor-excess silicon oil partition assay was employed.
- Equilibrium dissociation constants (Kd), association rates, and dissociation rates were measured.
- Binding affinities to various cell types were determined.
Main Results:
- Poliovirus type 1 Mahoney (PV1(M)) and poliovirus type 3 Sabin (PV3(S)) demonstrated similar binding to HeLa cells.
- Equilibrium dissociation constants were in the range of 1.6-2.1 x 10(-10) M.
- Association and dissociation rate constants were comparable between the virulent and avirulent strains.
Conclusions:
- Virulent and avirulent poliovirus strains exhibit nearly identical binding constants and rate constants to HeLa cells.
- Poliovirus binding affinity varies across different cell types, indicating receptor availability influences interaction strength.