Changes of pineal N-acetyltransferase activity in gamma irradiated rats

Male Wistar rats were exposed to whole body irradiation with 14.35 Gy gamma rays after the adaptation to light/dark cycle (LD 12:12). Three groups of rats were examined: A) rats irradiated in the night and placed in the 12 h LD cycle again, B) rats irradiated in the day-time and placed in the 12 h LD cycle, and C) rats irradiated in the night and kept in constant darkness. All analyses were carried out in the dark. Radiation enhanced the activity of pineal N-acetyltransferase 3-4 days after exposure in all groups, in the C group significantly on the 4th day. Different light regimens during and after irradiation did not to affect the activity of this key enzyme of melatonin synthesis substantially.