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Updated: Jul 21, 2026

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
Published on: November 10, 2011
Uptake of organic anions by hepatocyte monolayers: codiffusion versus facilitated dissociation
F J Burczynski1, J B Moran, Z S Cai
1Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Abstract:
We used the clearance ratio test to determine whether the presence of albumin enhances the uptake of palmitate by hepatocyte monolayers. If uptake is facilitated, then the ratio of palmitate clearance by hepatocytes in the presence of 600 and 300 microM albumin (i.e., Clt600/Clt300) will exceed the square root of the ratio of the unbound fractions at 600 and 300 microM albumin (i.e., square root of alpha 600/alpha 300). The latter value was determined using the heptane-water partitioning technique. The hepatocyte clearance ratio (0.92 +/- 0.06) exceeded the predicted value (0.80) by 15% (p < 0.05). The observed clearance rate also exceeded that predicted by the diffusion-reaction model.
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