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Human factor B. Complete cDNA sequence of the BF*S allele
J E Mejía1, I Jahn, H de la Salle
1Laboratory for Research in Immunology, Faculty of Medicine, Louis Pasteur University, Strasbourg, France.
Human Immunology
|January 1, 1994
Summary
Researchers present the first full-length coding sequence for the human complement factor B (BF) gene, specifically the major BF*S allele. This provides a crucial reference for understanding BF gene polymorphism and its role in the complement system.
Area of Science:
- Immunogenetics
- Molecular Biology
- Human Genetics
Background:
- The human complement factor B (BF) gene is located in the major histocompatibility complex class III region.
- Previous knowledge of the BF gene sequence was limited to partial studies, lacking a complete allele-specific sequence.
Purpose of the Study:
- To isolate and sequence complementary DNA (cDNA) clones of the major BF allele, BF*S.
- To provide an uninterrupted, allele-specific sequence of the entire coding region and 3' untranslated segment of BF cDNA.
Main Methods:
- Isolation and sequencing of cDNA clones derived from the BF*S allele.
- Comparative analysis of newly obtained sequence data with previously reported BF gene sequences.
Main Results:
- An uninterrupted, allele-specific sequence for the entire coding region and 3' untranslated segment of BF*S cDNA was determined.
- Comparison revealed several base substitutions compared to earlier published sequences, suggesting potential polymorphism.
Conclusions:
- The complete BF*S cDNA sequence offers a valuable resource for complement system research.
- Observed sequence differences highlight the importance of considering BF gene polymorphism in future studies.