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Related Experiment Videos

Alanine kinetics in humans during low-intensity exercise

F Carraro1, A Naldini, J M Weber

  • 1Shriners Burns Institute, Department of Surgery, University of Texas Medical Branch at Galveston 77550.

Medicine and Science in Sports and Exercise
|March 1, 1994
PubMed
Summary

During low-intensity exercise, whole body protein breakdown significantly increases, contributing more to alanine flux than previously thought. Pyruvate still plays a role, but protein catabolism accelerates.

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Area of Science:

  • Exercise Physiology
  • Human Metabolism
  • Biochemistry

Background:

  • Alanine flux increases during exercise, with pyruvate being a known contributor.
  • The precise role of protein breakdown in this increased alanine flux during exercise remains unclear.

Purpose of the Study:

  • To quantify the relative contributions of pyruvate and protein breakdown to the increased alanine flux during exercise.
  • To determine the impact of low-intensity exercise on whole-body protein catabolism.

Main Methods:

  • Utilized a primed, constant infusion of 15N-alanine and 1-13C-lactate tracers.
  • Measured alanine appearance, de novo synthesis, and release from protein breakdown in healthy subjects at rest and during treadmill exercise (45% VO2max).

Main Results:

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  • Total alanine appearance significantly increased during exercise.
  • Alanine derived from pyruvate increased, but its proportion of total alanine flux decreased.
  • Alanine derived from protein breakdown significantly increased, both in absolute terms and as a percentage of total alanine flux.

Conclusions:

  • Low-intensity exercise accelerates whole-body protein catabolism.
  • Protein breakdown becomes a more significant contributor to alanine flux during exercise.