[A model prokaryotic promotor. II. Function of designs with mutations in the "-35" region]

A series of DNA duplexes corresponding to the E. coli consensus promoter with random hexanucleotides in the -35 region have been synthesized and characterized. The library of recombinant plasmids with synthetic promoter-like inserts, oriented in the direction of the marker gal operon of the initial vector, have been obtained. Analysis of the library on indicator medium and S1 mapping of the in vivo transcription initiation points in several plasmids have shown that most constructions exhibit promoter properties, and the structures of their -35 regions may be varied.