Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two-minute miniprep method for plasmid DNA isolation

M C Tarczynski1, W J Meyer, J J Min

  • 15 Prime-->3 Prime, Inc., Boulder, CO 80303.

Biotechniques
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting the response of a long-distance migrant to changing environmental conditions in winter.

Ecology and evolution·2024
Same author

Head and neck burns are associated with long-term patient-reported dissatisfaction with appearance: A Burn Model System National Database study.

Burns : journal of the International Society for Burn Injuries·2019
Same author

Extracorporeal cardiopulmonary resuscitation in refractory intra-operative cardiac arrest: an observational study of 12-year outcomes in a single tertiary hospital.

Anaesthesia·2018
Same author

Predictor of fluid responsiveness in the 'grey zone': augmented pulse pressure variation through a temporary increase in tidal volume.

British journal of anaesthesia·2017
Same author

Microvascular reactivity and endothelial glycocalyx degradation when administering hydroxyethyl starch or crystalloid during off-pump coronary artery bypass graft surgery: a randomised trial.

Anaesthesia·2016
Same author

Annual Cost Of Conservative Treatment Of Supraventricular Tachycardias In Poland.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

INSTA-PREP is a rapid method for plasmid DNA preparation from bacterial cultures, yielding usable DNA in under two minutes. This technique is suitable for various molecular biology applications.

Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • Plasmid DNA isolation is a fundamental technique in molecular biology.
  • Current methods can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop an extremely rapid method for plasmid DNA preparation.
  • To provide plasmid DNA suitable for diverse molecular biology applications.

Main Methods:

  • Utilized a two-phase solution (phenol-chloroform-isoamyl alcohol and water/buffer) for direct DNA extraction.
  • Employed INSTA-PREP gel barrier material for efficient phase separation via centrifugation.
  • Processed bacterial cultures ranging from 1 to 3 mL.

Main Results:

  • Achieved plasmid DNA preparation in two minutes or less per sample.

Related Experiment Videos

  • Obtained yields of 3 to 10 micrograms of supercoiled plasmid DNA per mL of culture.
  • Demonstrated suitability of prepared plasmid DNA for restriction digestion, ligation, transformation, PCR, transfection, and sequencing.

    • Conclusions:

    • INSTA-PREP offers an exceptionally fast and efficient method for plasmid DNA isolation.
    • The prepared plasmid DNA is of high quality and compatible with a wide range of downstream molecular biology applications.