Related Experiment Video

Updated: Aug 26, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Secretion rate independent evaluation of IgM antibody avidity at the level of single immunocytes

G Doria, C Mancini, C DeLisi

    Journal of Immunology (Baltimore, Md. : 1950)
    |November 1, 1978
    PubMed

    The hemolytic plaque inhibition assay has been performed on spleen cells from mice immunized with TNP-HRBC to evaluate avidity of anti-TNP IgM antibodies. At different times after immunization direct plaques were inhibited by soluble TNP-EACA, TNP61-BGG, or anti-mu antiserum. Analysis of the inhibition data provided independent estimates of antibody avidity and secretion rate. Avidity was found to increase with time, to reach a maximum when the antibody response attained the peak value, and then to decline as the response was waning. There was a decrease followed by increase of the secretion rate concomitant with the rise and fall of the antibody response and avidity.

    More Related Videos

    Quantitative Measurement of Intrathecally Synthesized Proteins in Mice08:23

    Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

    Published on: November 29, 2019

    Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-1908:48

    Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

    Published on: February 16, 2022

    Related Concept Videos

    Antibody Structure01:10

    Antibody Structure

    Overview
    Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
    The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
    Antibodies consist of four polypeptide chains: two identical heavy...
    Affinity and Avidity01:41

    Affinity and Avidity

    Overview
    Immunological Memory01:23

    Immunological Memory

    Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
    What is Immunological Memory?
    Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature is...