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Related Experiment Videos

CD70 represents the human ligand for CD27

R Q Hintzen1, S M Lens, G Koopman

  • 1Department of Clinical (Viro-) Immunology, Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.

International Immunology
|March 1, 1994
PubMed
Summary

The newly discovered CD27 ligand (CD27L) is structurally similar to tumor necrosis factor (TNF) family members. Researchers confirmed that CD70 is the human CD27L by demonstrating cross-reactivity between CD70 and CD27L antibodies.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • CD27 ligand (CD27L) is a type II transmembrane molecule.
  • CD27L shares structural homology with tumor necrosis factor (TNF)-alpha, TNF-beta, lymphotoxin beta, CD40L, and CD30L.

Purpose of the Study:

  • To investigate the tissue distribution of CD27L.
  • To determine if CD70 represents the human CD27L.

Main Methods:

  • Utilized a CD27L specific monoclonal antibody (mAb) to examine tissue distribution.
  • Tested for cross-reactivity between CD70 and CD27L specific mAbs using transfected mouse fibroblasts.
  • Assessed the interference of CD70 mAbs with CD27L mAb binding.

Main Results:

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  • CD27L expression was found in B cells, thymic medullary stromal cells, and scattered T cells in various tissues.
  • CD70 mAbs reacted with CD27L-expressing cells.
  • CD70 mAbs interfered with the binding of CD27L mAbs.

    • Conclusions:

    • CD70 is identical to the human CD27L.
    • This finding clarifies the identity of the CD27 ligand, important for understanding immune cell interactions.