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An improved HPLC method to purify erythrocyte cholesterol for estimation of in vivo cholesterol synthesis using the
W W Wong1, D L Hachey, L L Clarke
1Department of Pediatrics, Baylor College of Medicine, Houston, TX.
Abstract:
We report on an improved HPLC method to purify erythrocyte cholesterol for use in measuring in vivo cholesterol synthesis from 2H2O. The new procedure uses a smaller compression cartridge column at a flow rate of 3 mL/min. The new method reduces by almost tenfold the amount of solvent required to maintain the analytical purity of the isolated cholesterol.