Organ perfusion equipment for the introduction and removal of cryoprotectants

A new system for perfusing organs with cryoprotective agents or other solutions is described. It represents the first system that combines the sophistication and versatility required for successful organ cryopreservation protocols with the experimental ease and reproducibility required for sustaining a long-term research or organ-processing effort with a high throughput of successfully perfused organs. Operation is simple, fully documented, and readily checked by visual means, and maintenance is not difficult. It appears that this system could be adapted for use with a wide variety of organ perfusion protocols and could lead to improved results and greater patient safety with less human effort.