Morulae at compaction and the pattern of protein synthesis in mouse embryos

Compaction of mouse embryos at the 8-cell stage causes a drastic change in cell form and in cell-to-cell contacts in 3-4 h. We have studied the effect of inhibitors of transcription (alpha-amanitin), DNA replication (aphidicolin) and compaction (cytochalasin D, EGTA, alpha-lactalbumin and Con A) on the pattern of protein synthesis using gel electrophoresis. Our results show that the pattern of protein synthesis is regulated principally by passage through S phase during each early cell cycle rather than by de novo transcription, while changes induced in cell form or contacts do not alter the pattern significantly.